Julianscott blogs

With our wealth of knowledge, we are able to provide a wide range of exosome services, such as characterization, purification, and isolation. We give the exact size distribution and concentration measurement of exosomes based on the extremely sensitive tunable resistive pulse sensing (TRPS) technology in order to better understand the properties of exosomes and aid the later therapeutic and diagnostic use.

Measuring the size distribution and concentration of exosomes is crucial when examining their cellular absorption and activity. Recently, the characterisation of exosome samples has made greater use of the TRPS technique, which is often used for particle size distribution and exosome concentration measurement. With the ability to perform high-throughput single particle measurements of colloids and/or biomolecular analytes driven through a size-tunable nanopore one at a time, TRPS is the most potent nanoparticle characterization technology currently on the market.

The method uses adjustable nanopore technology—which allows the passage of ionic current and particles to be managed by modifying the pore size—in conjunction with resistive pulse-sensing, which detects current flow via an aperture. A brief alteration in the ionic current flow, referred to as a blockade event, is observed when particles pass through a pore one at a time. The blockade magnitude is the amplitude of this change. After calibrating with a known standard, precise particle sizing can be obtained because blockade magnitude is proportionate to particle size.